HPLC Columns
Aegispak
01.

Product Description
02.

Product Specifications
03.

Analysis Data

High-quality, Stable, and High Reproducible
HPLC columns Aegispak Series

Chemical structure of the HPLC C18 column stationary phase.

Introduction

To minimize the effects of metal impurities and residual silanol groups present in silica gel, YJ Biochrom utilized its own repurified, chromatography-optimized silica gel, that has been re-purified by our company.

The surface of the purified silica gel was coated with a silicon polymer using chemical vapor deposition (CVD), and then functional groups were directly bonded to synthesize the packing material (gas-phase reaction).

To remove any remaining silanol groups that may still be present after the synthesis of the packing material, we conducted additional end-capping under high temperature and high pressure, minimizing the influence of residual silanol groups to the utmost extent.

The packing material, which has functional groups directly bonded to the silicon polymer (-Si-R), exhibits a subtle difference in separation patterns compared to conventional packing materials that bond functional groups to silanol groups (-Si-O-Si-R).

USP Class No. Particle Size(um) Pore Size(Ằ) Surface Area(m2/g) C(%) Usable pH range End Capping
Aegispak C18-F L1 3, 5 120 320 13 1.0~9.0 O
Aegispak C18-FA L1 3, 5 120 320 11 1.0~9.0 O
Aegispak C18-L L1 3, 5 100 330 14 1.0~9.0 O
Aegispak C18-LA L1 3, 5 100 330 13 1.0~9.0 O
Aegispak C8 L7 3, 5 100 330 8 1.0~9.0 O

Product Description

Aegispak C18-F

· This C18 HPLC column features relatively low hydrophobicity and low surface polarity.

· C18-F HPLC column is effective for the rapid analysis of highly hydrophobic compounds. (USP L1)

Aegispak C18-FA

· This is an Aegispak C18-F column that has been treated with strong acid for 6 days. It is highly resistant to acids. (USP L1)

Aegispak C18-L

· This is a HPLC column with excellent aqua characteristics (tested with 100% buffer mobile phase for over 100 hours).

· Aegispak C18-L HPLC column features high hydrophobicity and low surface polarity.

· As a general-purpose HPLC column with moderate retention characteristics, it is highly versatile for the analysis of both polar and hydrophobic compounds. (USP L1)

Aegispak C18-LA

· This is an Aegispak C18-L column that has been treated with strong acid for 6 days. It is a HPLC column with strong acid resistance. (USP L1)

Aegispak C8

· This C8 HPLC column features low hydrophobicity and high surface polarity.

· C8 HPLC column allows for faster analysis compared to C18, but effective separation requires a significant polarity difference between samples.

· C8 HPLC column is highly effective for rapid analyses, such as content testing of single substances. (USP L7)

Aegispak HPLC columns exhibits excellent reproducibility and consistent retention time (rt).

Retention Time Character Graph
(HPLC Condition)
Column C18, 5um
4.6 × 150mm
Mobile Phase 60% ACN
Flow rate 1.0 mL/min
Detector UV (254nm)
Temperature 35℃
Sample
  • Uracil
  • Methyl Benzoate
  • Toluene
  • Naphthalene

The Aegispak C18-L column shows minimal retention time (RT) variation even after flowing 100% buffer mobile phase for over 100 hours.

Aqua Character Graph
(HPLC Condition)
Column Aegispak C18-L 5um 4.6×150mm
Mobile Phase 20mM potassium phosphate buffer (pH 6.8)
Flow rate 1.0 mL/min
Temperature 40℃
Detector UV 254nm
Sample Adenine
Aegispak HPLC columns exhibit excellent lot reproducibility
HPLC chromatogram using Aegispak C18-L column, 5μm particle size, 4.6×150mm dimensions

< Aegispak C18-L 5um, 4.6×150mm >

HPLC chromatogram using Aegispak C18-F column, 5μm particle size, 4.6×150mm dimensions

<Aegispak C18-F 5um, 4.6×150mm>

3um 5um 10um (100Å)  10um (120Å)
Aegispak C18-F
Aegispak C18-FA
Aegispak C18-L
Aegispak C18-LA
Aegispak C8
Aegispak Silica
Horizontal=Inner Diameter(mm) / Vertical=Length(mm)
※ The length can be supplied as per your request within the available specifications
Classify 1.5 2.0 3.0 3.9 4.0 4.6 6.0 8.0 10 20
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Analysis of 9 kinds of Organic Acids Graph
(HPLC Condition)
Column Aegispak C18-L 5um 4.6×250mm
Mobile Phase 10mM Potassium phosphate Monobasic (pH 2.5) / Methanol = 98/2
Flow rate 0.8 mL/min
Temperature 30℃
Detector UV 220nm
Sample
  • Tartaric Acid
  • Glycolic Acid
  • Malic Acid
  • Malonic Acid
  • Lactic Acid
  • Acetic Acid
  • Maleic Acid
  • Citric Acid
  • Fumaric Acid
Preservatives Graph
(HPLC Condition)
Column Aegispak C18-L 5um 4.6×150mm
Mobile Phase A/B = 35/65
A: Acetonitrile
B: 50mM Ammonium Acetate Buffer (pH 4.6)
Flow rate 1.0 mL/min
Temperature 40℃
Detector UV 235nm
Sample
  • Benzoic Acid
  • Sorbic Acid
  • Dehydroacetic Acid
  • Methyl Paraben
  • Ethyl Paraben
  • Isopropyl Paraben
  • Propyl Paraben
  • Isobutyl Paraben
  • Butyl Paraben
Analysis of 16 kinds of Amino Acids Graph
(HPLC Condition)
Column Aegispak C18-L 5um 4.6×250mm
Mobile Phase (A) 40mM Sodium phosphate (dibasic) + 0.1% phosphoric acid
(B) ACN / Methanol / DW = 45 / 45 / 10
Flow rate 1.5 mL/min
Temperature 40℃