HPLC Columns
INNO
01.

Product Description
02.

Product Specifications
03.

Analysis Data

Precise, Stable and High Reproducible
HPLC Columns INNO Series

Chemical structure of a silicone-based filler used in INNO HPLC columns, showing Si–O backbone and organic side chains for enhanced chromatographic performance

Introduction

To reduce the impact of metal impurities present in silica gel, YJ Biochrom has re-purified high-purity silica gel for chromatography using its developed technology.

Functional groups have been directly bonded to the silanol groups of the silica gel to synthesize the filler. (-Si-O-Si-R)

Depending on the application, there are fillers that have been end-capped and those that have not.
Fillers for prep columns are also available (20 µm and 50 µm are available).

·

INNO HPLC Column Series

USP Class No. Particle Size(um) Pore Size(Ằ) Surface Area(m2/g) C(%) Usable pH range End Capping
INNO Column C18 L1 3, 3.5, 5, 10 120 320 18 2.0~8.0 O
INNO Column C18-HC L1 5 100 430 20 2.0~8.0 O
INNO Column C18-SB L1 3.5 120 320 17 1.0~11.0 O
INNO Column C18-SSB L1 5 120 320 18 1.0~11.0 O
INNO Column C18-SBA L1 3, 5 120 320 16 1.0~11.0 O
INNO Column C18-NE L1 3, 5 120 320 17 2.0~7.5 X
INNO Column C8 L7 5 120 320 12 2.0~8.0 O
INNO Column C8-SB L7 5 120 320 11 1.0~11.0 O
INNO Column C8-HC L7 5 100 430 12 2.0~8.0 O
INNO Column C8-NE L7 5 120 320 11 2.0~7.5 X
INNO Column C4 L26 5 120 320 8 2.0~8.0 O
INNO Column C1 L13 5 120 320 3 2.0~8.0 X
INNO Column Silica L3 3, 5, 10 100, 120 320 - 2.0~8.0 X
INNO Column Diol L20 5 120 320 - 2.0~8.0 X
INNO Column NH2 L8 3, 5, 10 120 320 6 2.0~8.0 X
INNO Column SCX L9 5 120 320 6 2.0~8.0 O
INNO Column SCX-L L9 5 100 320 5 2.0~8.0 O
INNO Column CN L10 5, 10 120 320 8 2.0~8.0 O
INNO Column Phenyl L11 5 120 320 11 2.0~8.0 O
INNO Column SAX L14 5 120 320 5 2.0~8.0 O
·

INNO-P Column Series

USP Class No. Particle Size(um) Pore Size(Ằ) Surface Area(m2/g) C(%) Usable pH range End Capping
INNO-P Column C18 L1 5 300 110 8 1.0~9.0 O
INNO-P Column C8 L7 5 300 110 5 1.0~9.0 O
INNO-P Column C4 L26 5 300 110 3 1.0~9.0 O
·

INNO-Mixed Column Series

USP Class No. Particle Size(um) Pore Size(Ằ) Surface Area(m2/g) C(%) Usable pH range End Capping
INNO Column C18/SCX (1/1) L## 5 120 320 12 2.0~8.0 O
NNO Column C18/SCX (5/1) L## 5 120 320 15 2.0~8.0 O
INNO Column C18/SAX (1/1) L## 5 120 320 13 2.0~8.0 O
INNO Column C18/SAX (5/1) L## 5 120 320 16 2.0~8.0 O

INNO Column C18

· This HPLC column has high hydrophobicity, which enhances the retention characteristics of hydrophobic substances and provides excellent separation.

· It is a column that has undergone three end-capping treatments at high temperatures after the introduction of the C18 group. (USP L1)

INNO Column C18-HC

· HC stands for High Carbon.

· It is a column with a carbon loading rate of 20%, which exhibits strong retention of hydrophobic substances.

· This HPLC column provides excellent separation of hydrophobic substances. (USP L1)

INNO Column C18-SB

· SB stands for Strong Base / Strong for Basic mobile phase.

· This HPLC column protects the silica gel surface with a multi-layer, providing excellent alkaline and acid resistance. (USP L1)

INNO Column C18-SSB

· This HPLC column further enhances alkaline resistance. (USP L1)

INNO Column C18-SBA

· This is the INNO HPLC Column C18-SB, which has been treated with strong acid for 6 days. It is a column with strong acid resistance. (USP L1)

INNO Column C18-NE

· NE stands for No Endcapping, indicating a column that has not undergone end-capping treatment after the C18 reaction.

· This HPLC column retains silanol groups, making it effective for the separation of polar substances. (USP L1)

INNO Column C8

· INNO C8 HPLC column exhibits greater retention characteristics and excellent separation compared to Aegispak C8.

· It is a column that has undergone three end-capping treatments at high temperatures after the introduction of the C8 group. (USP L7)

INNO Column C8-SB

· SB stands for Strong Base / Strong for Basic mobile phase.

· This HPLC column protects the silica gel surface with a multi-layer, providing excellent alkaline and acid resistance. (USP L7)

INNO Column C8-HC

· HC stands for High Carbon.

· It is a column with a carbon loading rate of 13%, which exhibits strong retention of hydrophobic substances.

· This HPLC column provides excellent separation of hydrophobic substances. (USP L7)

INNO Column C8-NE

· NE stands for No Endcapping, indicating a column that has not undergone end-capping treatment after the C8 reaction.

· INNO HPLC Column C8-NE retains silanol groups, making it effective for the separation of polar substances. (USP L7)

INNO Column C4

· This is a HPLC column that has been treated with butyl groups and undergone three end-capping treatments at high temperatures. (USP L26)

INNO Column Silica

· This is a HPLC column filled with ultra-high-purity silica gel that has been re-purified from high-purity silica gel. (USP L3)

INNO Column Diol

· This is a HPLC column that has been treated with glycidoxypropyl groups and undergone a ring-opening reaction. (USP L20)

INNO Column NH2

· This is a HPLC column that has been treated with aminopropyl groups. (USP L8)

INNO Column SCX

· This is a HPLC column that has been treated with sulfonic acid after the introduction of allylglycidyl ether. (USP L9)

INNO Column CN

· This is a HPLC column that has been treated with cyanopropyl groups and undergone end-capping. (USP L10)

INNO Column Phenyl

· This is a HPLC column that has been treated with phenylethyl groups and undergone end-capping. (USP L11)

INNO Column SAX

· This is a HPLC column that has been treated with strong basic quaternary ammonium anion exchange functional groups. (USP L14)

INNO-P Column C18

· This is a C18 column synthesized using silica gel with a pore size of 300 Å.

· It is suitable for the analysis of peptides and proteins. (USP L1)

INNO-P Column C8

· This is a C8 column synthesized using silica gel with a pore size of 300 Å.

· It is suitable for the analysis of peptides and proteins. (USP L7)

INNO-P Column C4

· This is a C4 column synthesized using silica gel with a pore size of 300 Å.

· It is suitable for the analysis of peptides and proteins. (USP L26)

INNO Column C18/SCX (1/1)

· This is a column with a 1:1 ratio of C18 and SCX functional groups.

· It helps with the retention of polar compounds. (USP L##)

INNO Column C18/SCX (5/1)

· This is a column with a 5:1 ratio of C18 and SCX functional groups.

· It is suitable for the simultaneous analysis of polar compounds with amine functional groups and non-polar compounds. (USP L##)

INNO Column C18/SAX (1/1)

· This is a column with a 1:1 ratio of C18 and SAX functional groups.

· It helps with the retention of polar compounds. (USP L##)

INNO Column C18/SAX (5/1)

· This is a column with a 5:1 ratio of C18 and SAX functional groups.

· It is suitable for the simultaneous analysis of polar compounds with carboxylic acid functional groups and non-polar compounds. (USP L##)

Retention Time Character Graph
(HPLC Condition)
Column C18, 5um
4.6 × 150mm
Mobile Phase 60% ACN
Flow rate 1.0 mL/min
Detector UV (254nm)
Temperature 35℃
Sample
  • Uracil
  • Methyl Benzoate
  • Toluene
  • Naphthalene
Retention Time Character Gragp
(HPLC Condition)
Column C18, 5um, 4.6×250mm
Mobile Phase 60% ACN
Flow rate 1.0 mL/min
Detector UV (254 nm)
Temperature 35℃
Sample
  • Uracil
  • Methyl Benzoate
  • Toluene
  • Naphthalene
Retention Time Character Gragp
(HPLC Condition)
Mobile Phase Hexane / EtOH = 85/15
Flow rate 1.0 mL/min
Detector UV (254nm)
Temperature 35℃
Sample
  • 2-nitrotoluene
  • Phenol
  • Resorcinol
  • Hydroquinone
Retention Time Character Gragp
(HPLC Condition)
Mobile Phase Hexane / EtOH = 98/2
Flow rate 1.0 mL/min
Detector UV (254nm)
Temperature 35℃
Sample
  • Toluene
  • p-Dinitrobenzene
Retention Time Character Gragp
(HPLC Condition)
Mobile Phase 0.2M (NH4)2H2PO4 (pH 3.5)
Flow rate 1.0 mL/min
Detector UV (254nm)
Temperature 35℃
Sample
  • Uracil
  • Thymine
  • Cytosine
Retention Time Character Gragp
(HPLC Condition)
Mobile Phase Acetonitrile / Water = 75/25
Flow rate 1.0 mL/min
Detector RID
Temperature 35℃
Sample
  • Fructose
  • Glucose
  • Saccharose
  • Maltose
The INNO HPLC Column C18-SB exhibits excellent acid and base resistance.
·

Acidic Condition

Acidic Condition Graph
(HPLC Condition)
Column INNO Column C18-SB 5um 4.6×150mm
Mobile Phase Acetonitrile / Water = 60 / 40
Flow rate 1.0 mL/min
Temperature 35℃
Detector UV 254nm
Sample n-Butyl benzoate
·

Alkaline Condition

Alkaline Condition Graph
(HPLC Condition)
Column INNO Column C18-SB 5um 4.6×150mm
Mobile Phase Acetonitrile / Water = 70 / 30
Flow rate 1.0 mL/min
Temperature 35℃
Detector UV 254nm
Sample Phenanthrene
INNO HPLC Columns exhibits excellent lot reproducibility

<INNO Column C18 5um, 4.6×150mm>

<INNO Column C18-SB 5um, 4.6×150mm>

<INNO Column Diol 5um, 4.6×150mm>

Classify 3um 3.5um 5um 10um (100Å)  10um (120Å)
INNO Column C18
INNO Column C18-HC
INNO Column C18-SB
INNO Column C18-SSB
INNO Column C18-SBA
INNO Column C18-NE
INNO Column C8
INNO Column C8-SB
INNO Column C8-HC
INNO Column C8-NE
INNO Column C4
INNO Column C1
INNO Column Silica
INNO Column Diol
INNO Column NH2
INNO Column SCX
INNO Column SCX-L
INNO Column CN
INNO Column Phenyl
INNO Column SAX
INNO-P Column C18
INNO-P Column C8
INNO-P Column C4
INNO Column C18/SCX(1/1)
INNO Column C18/SCX(5/1)
INNO Column C18/SAX(1/1)
INNO Column C18/SAX(5/1)
Horizontal=Inner Diameter(mm) / Vertical=Length(mm)
※ The length can be supplied as per your request within the available specifications
Classify 1.5 2.0 3.0 3.9 4.0 4.6 6.0 8.0 10 20
30
33
35
40
45
50
55
60
75
80
100
110
120
125
150
200
220
250
30
Vitamin B12 (LOQ) Graph
(HPLC Condition)
Column INNO Column C18 3um 4.6×150mm
Mobile Phase (A) Water
(B) Methanol
(B) 25% (0min) - 25% (15.0min) - 100% (15.1min) - 100% (20min) - 25% (20.1min) - 25% (30.0min) Gradient
Flow rate 0.8 mL/min
Temperature 35℃
Detector UV 360nm
Sample Vitamin B12
Water soluble Vitamins Graph
(HPLC Condition)
Column INNO Column C18 3um 4.6×150mm
Mobile Phase (A) 10mM KH2PO4 buffer (pH 3.0) / Acetonitrile = 98/2
(B) 10mM KH2PO4 buffer (pH 3.0) / Acetonitrile = 40/60
Flow rate 0.8 mL/min
Temperature 35℃
Detector UV 220nm
Sample
  • Thiamine
  • Ascorbic acid
  • Nicotinic acid
  • Pyridoxine
  • Pyridoxal
  • Nicotinamide
  • Pantothenic acid
  • Hydroxocobalamin
  • Cyanocobalamin
  • Folic acid
  • Riboflavin
  • Biotin